Defense response enhancement in strawberry via elicitors
Identifieur interne : 000259 ( Main/Exploration ); précédent : 000258; suivant : 000260Defense response enhancement in strawberry via elicitors
Auteurs : Gihan M. H. Hussein [Égypte] ; Tahany M. A. Abdel-Rahman [Égypte] ; A. H. Alwan [Égypte, Iraq]Source :
- 3 Biotech [ 2190-5738 ] ; 2016.
Abstract
In this study, cell-suspension culture of strawberry (
Url:
DOI: 10.1007/s13205-016-0431-9
PubMed: NONE
PubMed Central: 4909018
Affiliations:
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Le document en format XML
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<affiliation wicri:level="1"><nlm:aff id="Aff3">Biology Department, College of Science, AL-Mustansiriya University, Baghdad, Iraq</nlm:aff>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Defense response enhancement in strawberry via elicitors</title>
<author><name sortKey="Hussein, Gihan M H" sort="Hussein, Gihan M H" uniqKey="Hussein G" first="Gihan M. H." last="Hussein">Gihan M. H. Hussein</name>
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<author><name sortKey="Abdel Rahman, Tahany M A" sort="Abdel Rahman, Tahany M A" uniqKey="Abdel Rahman T" first="Tahany M. A." last="Abdel-Rahman">Tahany M. A. Abdel-Rahman</name>
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<wicri:noRegion>Giza</wicri:noRegion>
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<author><name sortKey="Alwan, A H" sort="Alwan, A H" uniqKey="Alwan A" first="A. H." last="Alwan">A. H. Alwan</name>
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<series><title level="j">3 Biotech</title>
<idno type="eISSN">2190-5738</idno>
<imprint><date when="2016">2016</date>
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<front><div type="abstract" xml:lang="en"><p>In this study, cell-suspension culture of strawberry (<italic>Fragaria</italic>
× <italic>ananassa</italic>
), cultivars Camarosa, and Sweet Charlie has been established. Embryogenic callus was induced by incubating the in vitro juvenile leaf explants on medium, containing 2-mg/l picloram at dark. Suspension culture was initiated from 4-week-old embryogenic calli in the liquid MS medium with 1-mg/l 2,4-D and 2-mg/l picloram. Suspension culture was maintained by sub-culturing each 3 weeks into a fresh medium. At week 9 after third sub-cultures, torpedo and cotyledonary embryo stages were observed. Embryos were then developed into shoots on medium 1 mg/l of each BA and IBA. Obtained shoots were successfully rooted on 1-mg/ml GA3, 0.5-mg/ml BA, and 1-mg/ml IBA. To enhance the resistance availability in strawberry plants, elicitation was applied by adding the JA and SA elicitors to the suspension culture with two doses (0.5 and 1 mM) individually and in combination, in addition to the fungal homogenate of <italic>Macrophomina phasiolena</italic>
at concentration of 10<sup>6</sup>
spor/ml. The fa<italic>wrky-1-Camarosa</italic>
gene, which has defense-related function, was detected in the different elicited strawberry tissues and isolated via RT-PCR. The isolated gene was submitted to GenBank with accession number (KX096885).</p>
</div>
</front>
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</biblStruct>
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</back>
</TEI>
<affiliations><list><country><li>Iraq</li>
<li>Égypte</li>
</country>
</list>
<tree><country name="Égypte"><noRegion><name sortKey="Hussein, Gihan M H" sort="Hussein, Gihan M H" uniqKey="Hussein G" first="Gihan M. H." last="Hussein">Gihan M. H. Hussein</name>
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<name sortKey="Abdel Rahman, Tahany M A" sort="Abdel Rahman, Tahany M A" uniqKey="Abdel Rahman T" first="Tahany M. A." last="Abdel-Rahman">Tahany M. A. Abdel-Rahman</name>
<name sortKey="Alwan, A H" sort="Alwan, A H" uniqKey="Alwan A" first="A. H." last="Alwan">A. H. Alwan</name>
</country>
<country name="Iraq"><noRegion><name sortKey="Alwan, A H" sort="Alwan, A H" uniqKey="Alwan A" first="A. H." last="Alwan">A. H. Alwan</name>
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</country>
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</record>
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